Production of phenolic compounds from Spirulina maxima microalgae and its protective effects in vitro toward hepatotoxicity model

نویسندگان

  • Hanaa H. Abd El-Baky
  • Farouk K. El Baz
  • Gamal S. El-Baroty
چکیده

This study illustrates the process of enhancing phenolics synthesis in Spirulina maxima grown in Zarrouk’s medium supplemented with sodium nitrate (NaNO3) and or phenylalanine (L-PA), attaining highest production obtained in medium containing 3.77 g.L NaNO3 and 100 mgL -1 L-PA. HPLC profile showed the presence of phenolic acids and flavonoids predominantly with gallate, chlorogenate, cinnamate, pinostrobate and p-OH-benzoates. The protective action of Spirulina phenolic compounds (SPC) against CCl4-induced in vitro hepato-toxicity symptoms like microsomal lipid peroxidation and hydroxyl radical formation was studied. SPC exhibited antioxidant effects on DPPH radical scavenging with IC50 values ranging from 23.22 to 35.62 μgmL -1 and inhibit CCl4 induced lipid peroxidation in hepato-microsoms model, in dose-depended manner. Their protective potential was comparable to that of standard phenolic antioxidants such as BHT, BHA and – tocopherol (IC50 values ranged from 13.22 to 23.62 μgmL ).

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تاریخ انتشار 2009